"The Interuniversity Consortium for Magnetic Resonances of Metal Protein (CIRMMP) operates jointly with the Magnetic Resonances Centre (CERM), sharing its structures and facilities. The Consortium was founded in 1994 between the Universities of Florence, Siena and Bologna to develop and coordinate resources in the specific field of the study of metalloproteins and metalloenzymes, involved in numerous biological processes, through magnetic resonance techniques in the first place, among the most advanced in the world, accompanied by other techniques (EPR, calorimetry, CD, microscopy, mass spectrometry, X-rays, etc..) available in laboratories supporting the Consortium.
"Tools for extracting function information from metalloproteins databases, creation of metalloproteins databases.
Biophysical characterization and protein structure determination. Protein-protein ;protein-DNA,protein-small molecule interaction studies "
Description: "The center is equipped with NMR at 950 MHz, 900 MHz, 850 MHz WB, 800 MHz, 700 MHz WB, 2 x 700 MHz, 2 x 600 MHz, 500 MHz and 400 MHz, as well as other routine instruments, as well as a relaxometer. The large number of instruments available allows the combination of all NMR investigation methods. All the high resolution instruments are digital spectrometers of the Bruker AVANCE series, most of them are equipped with CryoProbe to improve their sensitivity and with different electronic configurations (number of channels, lock-switches, boards, etc.) to satisfy the most different experimental needs. Further details:
Studies for the structural characterization of biological macromolecules, screening of drug candidates, studies for the design of innovative vaccines, characterization of metabolic profiles of biological fluids, food quality, material characterization.
External access conditions: The request for the use of the instruments, available both at national and international level, is made through an online form for the request of machine time, to be completed both from the qualitative (spectrometer required and type of experiments) and quantitative (number of days required) point of view. Applications are evaluated by a committee composed of internal staff and external experts. The form is available here: http://www.cerm.unifi.it/static/access/access.html